RAPD variation within and among natural populations of trembling aspen (Populus tremuloides Michx.) from Alberta.
Identifieur interne : 004A64 ( Main/Exploration ); précédent : 004A63; suivant : 004A65RAPD variation within and among natural populations of trembling aspen (Populus tremuloides Michx.) from Alberta.
Auteurs : F C Yeh [Canada] ; D K Chong ; R C YangSource :
- The Journal of heredity [ 0022-1503 ]
Descripteurs français
- KwdFr :
- ADN des plantes (isolement et purification), Alberta (MeSH), Amorces ADN (MeSH), Arbres (génétique), Données de séquences moléculaires (MeSH), Géographie (MeSH), Opéron (MeSH), Phylogenèse (MeSH), Polymorphisme génétique (MeSH), Réaction de polymérisation en chaîne (MeSH), Séquence nucléotidique (MeSH), Variation génétique (MeSH).
- MESH :
- génétique : Arbres.
- isolement et purification : ADN des plantes.
- Alberta, Amorces ADN, Données de séquences moléculaires, Géographie, Opéron, Phylogenèse, Polymorphisme génétique, Réaction de polymérisation en chaîne, Séquence nucléotidique, Variation génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : DNA, Plant.
- chemical : DNA Primers.
- geographic : Alberta.
- genetics : Trees.
- Base Sequence, Genetic Variation, Geography, Molecular Sequence Data, Operon, Phylogeny, Polymerase Chain Reaction, Polymorphism, Genetic.
Abstract
This article described the population structure of trembling aspen (Populus tremuloides Michx.) in Alberta, a dioecious tree with continuous and wide distribution, and with a primary mode of reproduction through suckering. We studied random amplified polymorphic DNA (RAPD) variation in 249 trees from eight natural populations. Trees within a population were a minimum of 200 m apart to decrease the risk of sampling ramets of a single ortet. Of a total of 28 amplified RAPD products (bands) from five random oligonucleotide primers, the frequencies at seven (25%) were heterogeneous across populations and the percentage of polymorphism averaged 90.2 per population. Estimates of Shannon's phenotypic diversity index ranged between 0.58 and 0.69 among populations, averaging 0.65. There were 246 multiband phenotypes among the 249 trees; three were each shared by two trees from different populations and the remaining 243 were unique. Thus, trees within populations probably were different clones. Analysis of molecular variance partitioned the RAPD variation into the among- and within-population components. The within-population component accounted for 97.4% of the variation and was significantly different from zero at the 2% level of probability. The among-population component, although accounting for only 2.6% of the variation, was significantly different from zero at the 1% level of probability. Pairwise tests for the homogeneity of the RAPD variance between populations suggested significant divergences among 18 of the 28 (64%) population pairs.
DOI: 10.1093/oxfordjournals.jhered.a111620
PubMed: 8568211
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">RAPD variation within and among natural populations of trembling aspen (Populus tremuloides Michx.) from Alberta.</title><author><name sortKey="Yeh, F C" sort="Yeh, F C" uniqKey="Yeh F" first="F C" last="Yeh">F C Yeh</name><affiliation wicri:level="3"><nlm:affiliation>Department of Renewable Resources, University of Alberta, Edmonton, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Renewable Resources, University of Alberta, Edmonton</wicri:regionArea><placeName><settlement type="city">Edmonton</settlement><region type="state">Alberta</region></placeName></affiliation></author><author><name sortKey="Chong, D K" sort="Chong, D K" uniqKey="Chong D" first="D K" last="Chong">D K Chong</name></author><author><name sortKey="Yang, R C" sort="Yang, R C" uniqKey="Yang R" first="R C" last="Yang">R C Yang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995 Nov-Dec</date><idno type="RBID">pubmed:8568211</idno><idno type="pmid">8568211</idno><idno type="doi">10.1093/oxfordjournals.jhered.a111620</idno><idno type="wicri:Area/Main/Corpus">004A67</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004A67</idno><idno type="wicri:Area/Main/Curation">004A67</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004A67</idno><idno type="wicri:Area/Main/Exploration">004A67</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">RAPD variation within and among natural populations of trembling aspen (Populus tremuloides Michx.) from Alberta.</title><author><name sortKey="Yeh, F C" sort="Yeh, F C" uniqKey="Yeh F" first="F C" last="Yeh">F C Yeh</name><affiliation wicri:level="3"><nlm:affiliation>Department of Renewable Resources, University of Alberta, Edmonton, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Renewable Resources, University of Alberta, Edmonton</wicri:regionArea><placeName><settlement type="city">Edmonton</settlement><region type="state">Alberta</region></placeName></affiliation></author><author><name sortKey="Chong, D K" sort="Chong, D K" uniqKey="Chong D" first="D K" last="Chong">D K Chong</name></author><author><name sortKey="Yang, R C" sort="Yang, R C" uniqKey="Yang R" first="R C" last="Yang">R C Yang</name></author></analytic><series><title level="j">The Journal of heredity</title><idno type="ISSN">0022-1503</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alberta (MeSH)</term><term>Base Sequence (MeSH)</term><term>DNA Primers (MeSH)</term><term>DNA, Plant (isolation & purification)</term><term>Genetic Variation (MeSH)</term><term>Geography (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Operon (MeSH)</term><term>Phylogeny (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Polymorphism, Genetic (MeSH)</term><term>Trees (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (isolement et purification)</term><term>Alberta (MeSH)</term><term>Amorces ADN (MeSH)</term><term>Arbres (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Géographie (MeSH)</term><term>Opéron (MeSH)</term><term>Phylogenèse (MeSH)</term><term>Polymorphisme génétique (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Alberta</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arbres</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN des plantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Genetic Variation</term><term>Geography</term><term>Molecular Sequence Data</term><term>Operon</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Polymorphism, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alberta</term><term>Amorces ADN</term><term>Données de séquences moléculaires</term><term>Géographie</term><term>Opéron</term><term>Phylogenèse</term><term>Polymorphisme génétique</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This article described the population structure of trembling aspen (Populus tremuloides Michx.) in Alberta, a dioecious tree with continuous and wide distribution, and with a primary mode of reproduction through suckering. We studied random amplified polymorphic DNA (RAPD) variation in 249 trees from eight natural populations. Trees within a population were a minimum of 200 m apart to decrease the risk of sampling ramets of a single ortet. Of a total of 28 amplified RAPD products (bands) from five random oligonucleotide primers, the frequencies at seven (25%) were heterogeneous across populations and the percentage of polymorphism averaged 90.2 per population. Estimates of Shannon's phenotypic diversity index ranged between 0.58 and 0.69 among populations, averaging 0.65. There were 246 multiband phenotypes among the 249 trees; three were each shared by two trees from different populations and the remaining 243 were unique. Thus, trees within populations probably were different clones. Analysis of molecular variance partitioned the RAPD variation into the among- and within-population components. The within-population component accounted for 97.4% of the variation and was significantly different from zero at the 2% level of probability. The among-population component, although accounting for only 2.6% of the variation, was significantly different from zero at the 1% level of probability. Pairwise tests for the homogeneity of the RAPD variance between populations suggested significant divergences among 18 of the 28 (64%) population pairs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8568211</PMID><DateCompleted><Year>1996</Year><Month>03</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>12</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-1503</ISSN><JournalIssue CitedMedium="Print"><Volume>86</Volume><Issue>6</Issue><PubDate><MedlineDate>1995 Nov-Dec</MedlineDate></PubDate></JournalIssue><Title>The Journal of heredity</Title><ISOAbbreviation>J Hered</ISOAbbreviation></Journal><ArticleTitle>RAPD variation within and among natural populations of trembling aspen (Populus tremuloides Michx.) from Alberta.</ArticleTitle><Pagination><MedlinePgn>454-60</MedlinePgn></Pagination><Abstract><AbstractText>This article described the population structure of trembling aspen (Populus tremuloides Michx.) in Alberta, a dioecious tree with continuous and wide distribution, and with a primary mode of reproduction through suckering. We studied random amplified polymorphic DNA (RAPD) variation in 249 trees from eight natural populations. Trees within a population were a minimum of 200 m apart to decrease the risk of sampling ramets of a single ortet. Of a total of 28 amplified RAPD products (bands) from five random oligonucleotide primers, the frequencies at seven (25%) were heterogeneous across populations and the percentage of polymorphism averaged 90.2 per population. Estimates of Shannon's phenotypic diversity index ranged between 0.58 and 0.69 among populations, averaging 0.65. There were 246 multiband phenotypes among the 249 trees; three were each shared by two trees from different populations and the remaining 243 were unique. Thus, trees within populations probably were different clones. Analysis of molecular variance partitioned the RAPD variation into the among- and within-population components. The within-population component accounted for 97.4% of the variation and was significantly different from zero at the 2% level of probability. The among-population component, although accounting for only 2.6% of the variation, was significantly different from zero at the 1% level of probability. Pairwise tests for the homogeneity of the RAPD variance between populations suggested significant divergences among 18 of the 28 (64%) population pairs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yeh</LastName><ForeName>F C</ForeName><Initials>FC</Initials><AffiliationInfo><Affiliation>Department of Renewable Resources, University of Alberta, Edmonton, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chong</LastName><ForeName>D K</ForeName><Initials>DK</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>R C</ForeName><Initials>RC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Hered</MedlineTA><NlmUniqueID>0375373</NlmUniqueID><ISSNLinking>0022-1503</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000416" MajorTopicYN="N" Type="Geographic">Alberta</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005843" MajorTopicYN="N">Geography</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009876" MajorTopicYN="N">Operon</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="Y">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011110" MajorTopicYN="Y">Polymorphism, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>11</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>11</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8568211</ArticleId><ArticleId IdType="doi">10.1093/oxfordjournals.jhered.a111620</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Alberta</li></region><settlement><li>Edmonton</li></settlement></list><tree><noCountry><name sortKey="Chong, D K" sort="Chong, D K" uniqKey="Chong D" first="D K" last="Chong">D K Chong</name><name sortKey="Yang, R C" sort="Yang, R C" uniqKey="Yang R" first="R C" last="Yang">R C Yang</name></noCountry><country name="Canada"><region name="Alberta"><name sortKey="Yeh, F C" sort="Yeh, F C" uniqKey="Yeh F" first="F C" last="Yeh">F C Yeh</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004A64 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004A64 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:8568211
|texte= RAPD variation within and among natural populations of trembling aspen (Populus tremuloides Michx.) from Alberta.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:8568211" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |